A complete of 297 fungal strains ended up isolated and cultivated over the 24-perfectly plate within the compact-scale assay. As proven in Fig. 2, most fungal isolates were in the position to secrete cellulases, and full protein contents had been lower or similar to that of A. aculeatus
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BCC199. Among the them, pressure AG500 exhibited exceptional cellulase activity and protein manufacturing soon after tests depending on equally the first and secondary screening techniques.
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Immediately after the first and secondary screenings, powerful fungal strains have been recognized by molecular info. DNA extraction from the mycelium on PDA was executed applying the strategy described by [29] with a few modifications.
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